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PDA-HP/NIR treatment suppresses glial activation and promotes a shift toward anti-inflammatory polarization following SCI. (A) Representative fluorescence images and quantification showing <t>GFAP</t> (green) and IBA1 (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (B, C). Data are presented as mean ± SD ( n = 6, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (D) Representative fluorescence images and quantification showing Arg-1 (green) and iNOS (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (E, F). Data are presented as mean ± SD ( n = 6, ∗ p = 0.011, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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PDA-HP/NIR treatment suppresses glial activation and promotes a shift toward anti-inflammatory polarization following SCI. (A) Representative fluorescence images and quantification showing <t>GFAP</t> (green) and IBA1 (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (B, C). Data are presented as mean ± SD ( n = 6, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (D) Representative fluorescence images and quantification showing Arg-1 (green) and iNOS (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (E, F). Data are presented as mean ± SD ( n = 6, ∗ p = 0.011, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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PDA-HP/NIR treatment suppresses glial activation and promotes a shift toward anti-inflammatory polarization following SCI. (A) Representative fluorescence images and quantification showing <t>GFAP</t> (green) and IBA1 (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (B, C). Data are presented as mean ± SD ( n = 6, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (D) Representative fluorescence images and quantification showing Arg-1 (green) and iNOS (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (E, F). Data are presented as mean ± SD ( n = 6, ∗ p = 0.011, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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PDA-HP/NIR treatment suppresses glial activation and promotes a shift toward anti-inflammatory polarization following SCI. (A) Representative fluorescence images and quantification showing <t>GFAP</t> (green) and IBA1 (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (B, C). Data are presented as mean ± SD ( n = 6, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (D) Representative fluorescence images and quantification showing Arg-1 (green) and iNOS (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (E, F). Data are presented as mean ± SD ( n = 6, ∗ p = 0.011, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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PDA-HP/NIR treatment suppresses glial activation and promotes a shift toward anti-inflammatory polarization following SCI. (A) Representative fluorescence images and quantification showing GFAP (green) and IBA1 (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (B, C). Data are presented as mean ± SD ( n = 6, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (D) Representative fluorescence images and quantification showing Arg-1 (green) and iNOS (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (E, F). Data are presented as mean ± SD ( n = 6, ∗ p = 0.011, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Dual-responsive PDA-HP hydrogel enables mitochondria-targeted mild photothermal therapy for spinal cord repair

doi: 10.1016/j.mtbio.2026.102783

Figure Lengend Snippet: PDA-HP/NIR treatment suppresses glial activation and promotes a shift toward anti-inflammatory polarization following SCI. (A) Representative fluorescence images and quantification showing GFAP (green) and IBA1 (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (B, C). Data are presented as mean ± SD ( n = 6, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (D) Representative fluorescence images and quantification showing Arg-1 (green) and iNOS (red) staining of spinal cord sections in each group. Scale bars are 10 μm. Quantification of mean fluorescence intensity is shown in (E, F). Data are presented as mean ± SD ( n = 6, ∗ p = 0.011, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Samples were then incubated overnight at 4 °C with the following primary antibodies: GFAP (astrocyte marker, 1:500, Proteintech, China), Iba1 (microglia marker, 1:500, Proteintech, China), Arg-1 (M2 phenotype marker, 1:500, Proteintech, China), iNOS (M1 phenotype marker, 1:500, Proteintech, China).

Techniques: Activation Assay, Fluorescence, Staining